SEQUENCE STUDIES OF POLIOVIRUS RNA .4. NUCLEOTIDE-SEQUENCE COMPLEXITIES OF POLIOVIRUS TYPE-1, TYPE-2 AND 2 TYPE-1 DEFECTIVE INTERFERING PARTICLES RNAS, AND FINGERPRINT OF THE POLIOVIRUS TYPE-3 GENOME

被引：57

作者：

LEE, YF ^{[1
]}

KITAMURA, N ^{[1
]}

NOMOTO, A ^{[1
]}

WIMMER, E ^{[1
]}

机构：

[1] SUNY STONY BROOK, SCH BAS HLTH SCI, DEPT MICROBIOL, STONY BROOK, NY 11794 USA

来源：

JOURNAL OF GENERAL VIROLOGY | 1979年 / 44卷 / AUG期

关键词：

D O I：

10.1099/0022-1317-44-2-311

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 32P-labelled genomes of poliovirus type 1, 2 and 3 have been digested with RNase T1 and the products separated by two-dimensional gel electrophoresis. All three fingerprints differ in the separation pattern of the large oligonucleotides. The molar yields of the large RNase T1-resistant oligonucleotides of type 1 and type 2 RNA of poliovirus RNA are close to one. By comparing the yields of these oligonucleotides to the amount of RNA from which they originated, the chain length of type 1 poliovirus RNA was found to be 7851 ± 567 nucleotides (mol. wt. 2.66 ± 0.19 x 106) and that of poliovirus type 2, 8181 ± 578 nucleotides (mol. wt. 2.77 ± 0.19 x 106). The chain length of two defective interfering particle (DI) RNAs of poliovirus type 1 were determined to be 7042 ± 999 nucleotides for DI(1) and 6639 ± 674 nucleotides for DI(2).

引用

页码：311 / 322

页数：12

共 39 条

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