LEUKOREGULIN DOWN-REGULATES TYPE-I COLLAGEN MESSENGER-RNA LEVELS AND PROMOTER ACTIVITY IN HUMAN DERMAL FIBROBLASTS, AND COUNTERACTS THE UP-REGULATION ELICITED BY TRANSFORMING GROWTH-FACTOR-BETA

被引：10

作者：

MAUVIEL, A

EVANS, CH

UITTO, J

机构：

[1] THOMAS JEFFERSON UNIV, JEFFERSON MED COLL, DEPT DERMATOL, PHILADELPHIA, PA 19107 USA

[2] THOMAS JEFFERSON UNIV, JEFFERSON INST MOLEC MED, MOLEC DERMATOL SECT, PHILADELPHIA, PA 19107 USA

[3] THOMAS JEFFERSON UNIV, JEFFERSON MED COLL, DEPT BIOCHEM & MOLEC BIOL, PHILADELPHIA, PA 19107 USA

[4] NCI, DIV CANC ETIOL, TUMOR BIOL SECT, BIOL LAB, BETHESDA, MD 20892 USA

来源：

BIOCHEMICAL JOURNAL | 1992年 / 284卷

关键词：

D O I：

10.1042/bj2840629

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leukoregulin (LR), a T-cell-derived growth factor, modulates fibroblast functions in vitro [Mauviel, Redini, Hartmann, Loyau & Pujol (1991) J. Cell Biol. 113, 1455-14621. In the present study, incubation of human dermal fibroblasts with LR (0.1-2 units/ml) resulted in decreases in the mRNA steady-state levels for alpha-1(I), alpha-2(I) and alpha-1(III), but not alpha-2(V), collagen genes. LR also down-regulated alpha-2(I) collagen promoter activity in transient cell transfections of control cells as well as those incubated with transforming growth factor-beta, a potent up-regulator of collagen type I gene expression. Thus LR is a strong inhibitor of type I collagen gene expression, acting at the level of transcription.

引用

页码：629 / 632

页数：4

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