CHEMICAL MODIFICATION OF LIPASE WITH A COMB-SHAPED SYNTHETIC COPOLYMER OF POLYOXYETHYLENE ALLYL METHYL DIETHER AND MALEIC-ANHYDRIDE

被引：18

作者：

HIROTO, M ^{[1
]}

MATSUSHIMA, A ^{[1
]}

KODERA, Y ^{[1
]}

SHIBATA, Y ^{[1
]}

INADA, Y ^{[1
]}

机构：

[1] TOIN UNIV YOKOHAMA,DEPT MAT SCI & TECHNOL,1614 KUROGANE-CHO,MIDORI KU,YOKOHAMA 225,JAPAN

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 07期

关键词：

D O I：

10.1007/BF01023940

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lipase from Pseudomonas fluorescens was coupled with a copolymer of polyoxyethylene allyl methyl diether and maleic anhydride, activated PM. The PM-lipase became soluble and active in organic solvents, and also heat stable. It catalyzed the ester synthesis in benzene and ester hydrolysis in an aqueous system with high enzymic activity.

引用

页码：559 / 564

页数：6

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