SEPARATION OF FRAGMENTS UP TO 570 BASES IN LENGTH BY USE OF 6-PERCENT T-NON-CROSS-LINKED POLYACRYLAMIDE FOR DNA-SEQUENCING IN CAPILLARY ELECTROPHORESIS

被引：63

作者：

BEST, N ^{[1
]}

ARRIAGA, E ^{[1
]}

CHEN, DY ^{[1
]}

DOVICHI, NJ ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON T6G 2G2,AB,CANADA

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 22期

关键词：

D O I：

10.1021/ac00094a031

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Non-cross-linked polyacrylamide is a very convenient medium for the separation of DNA sequencing fragments in capillary electrophoresis. We demonstrate DNA sequencing with this matrix at an electric field of 200 V/cm and at room temperature. Resolution is observed to decrease exponentially with fragment length. Fragments 570 bases in length generate a resolution of 0.5, which is adequate for sequence identification.

引用

页码：4063 / 4067

页数：5

共 49 条

[1] USE OF LIQUID POLYACRYLAMIDE IN ELECTROPHORESIS .2. RELATIONSHIP BETWEEN GEL VISCOSITY AND MOLECULAR SIEVING
BODE, HJ
[J]. ANALYTICAL BIOCHEMISTRY, 1977, 83 (02) : 364 - 371
[2] A STUDY OF ELECTROPHORETIC MOBILITY OF DNA IN AGAROSE AND POLYACRYLAMIDE GELS
CALLADINE, CR
COLLIS, CM
DREW, HR
MOTT, MR
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1991, 221 (03) : 981 - 1005
[3] LOW-COST, HIGH-SENSITIVITY LASER-INDUCED FLUORESCENCE DETECTION FOR DNA SEQUENCING BY CAPILLARY GEL-ELECTROPHORESIS
CHEN, DY
SWERDLOW, HP
HARKE, HR
ZHANG, JZ
DOVICHI, NJ
[J]. JOURNAL OF CHROMATOGRAPHY, 1991, 559 (1-2): : 237 - 246
[4] SIMPLE LASER-INDUCED FLUORESCENCE DETECTOR FOR SULFORHODAMINE-101 IN A CAPILLARY ELECTROPHORESIS SYSTEM - DETECTION LIMITS OF 10 YOCTOMOLES OR 6 MOLECULES
CHEN, DY
ADELHELM, K
CHENG, XL
DOVICHI, NJ
[J]. ANALYST, 1994, 119 (02) : 349 - 352
[5] 2-LABEL PEAK-HEIGHT ENCODED DNA SEQUENCING BY CAPILLARY GEL-ELECTROPHORESIS - 3 EXAMPLES
CHEN, DY
HARKE, HR
DOVICHI, NJ
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (18) : 4873 - 4880
[6] SUBATTOMOLE AMINO-ACID ANALYSIS BY CAPILLARY ZONE ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE
CHENG, YF
DOVICHI, NJ
[J]. SCIENCE, 1988, 242 (4878) : 562 - 564
[7] INTERACTION OF CAPILLARY ZONE ELECTROPHORESIS WITH A SHEATH FLOW CUVETTE DETECTOR
CHENG, YF
WU, SL
CHEN, DY
DOVICHI, NJ
[J]. ANALYTICAL CHEMISTRY, 1990, 62 (05) : 496 - 503
[8] CAPILLARY ELECTROPHORESIS OF MACROMOLECULES IN SYRUPY SOLUTIONS - FACTS AND MISFACTS
CHIARI, M
NESI, M
FAZIO, M
RIGHETTI, PG
[J]. ELECTROPHORESIS, 1992, 13 (9-10) : 690 - 697
[9] SEPARATION AND ANALYSIS OF DNA-SEQUENCE REACTION-PRODUCTS BY CAPILLARY GEL-ELECTROPHORESIS
COHEN, AS
NAJARIAN, DR
KARGER, BL
[J]. JOURNAL OF CHROMATOGRAPHY, 1990, 516 (01): : 49 - 60
[10] ATTOGRAM DETECTION LIMIT FOR AQUEOUS DYE SAMPLES BY LASER-INDUCED FLUORESCENCE
DOVICHI, NJ
MARTIN, JC
JETT, JH
KELLER, RA
[J]. SCIENCE, 1983, 219 (4586) : 845 - 847

← 1 2 3 4 5 →