USE OF GUANIDINE THIOCYANATE TO FRACTIONATE DNA-LIKE RNA ADSORBED ON METHYLATED BOVINE ALBUMIN KIESELGUHR COLUMNS

The strong binding of most of the DNA-like RNA from mammalian cells to methylated albumin kieselguhr forms the basis of a method for quantitating and fractionating it. A concentration gradient of guanidine thiocyanate will elute more than 90 % of the tenaciously bound D-RNA. Contamination of TD-RNA by isotope present in rRNA is minimal, and this is preferentially eluted first, in a sharply defined peak eluted by 0.1-0.7 M guanidine thiocyanate. The method exhibits differences in the elution patterns of TD-RNA derived from different cell types. © 1969.