DISTRIBUTION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS SEROTYPES IN PERIODONTAL HEALTH AND DISEASE

被引：164

作者：

ASIKAINEN, S

LAI, CH

ALALUUSUA, S

SLOTS, J

机构：

[1] Department of Periodontology, University of Pennsylvania, Philadelphia, Pennsylvania

[2] Department of Periodontics, University of Pennsylvania, Philadelphia, Pennsylvania

[3] Department of Pedodontics and Orthodontics, University of Helsinki, Helsinki

[4] Department of Periodontology, University of Southern California, Los Angeles, California

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 6卷 / 02期

关键词：

ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; SEROTYPE; INDIRECT IMMUNOFLUORESCENCE; IMMUNODIFFUSION; PERIODONTAL DISEASE;

D O I：

10.1111/j.1399-302X.1991.tb00462.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

A total of 177 Actinobacillus actinomycetemcomitans isolates from 136 periodontally healthy or diseased subjects were serotyped by indirect immunofluorescence and/or immunodiffusion assays. Serotype-specific rabbit antisera against A. actinomycetemcomitans serotypes a, b and c were used. All 3 serotypes were commonly found in the study subjects. Serotype b was dominant in subjects with periodontal disease and serotype c was the most common serotype in the healthy subjects. In the immunofluorescence assay, when 85 isolates were cultured anaerobically and fixed in acetone, or cultured aerobically in 10% CO2 and heat-fixed, 60 isolates revealed the same serotypes. The remaining 25 isolates reacted with 2 of the serotype-determining reagents. In the immunodiffusion assay, 22 of these 25 isolates reacted with one antiserum only. These results suggest differences in the distribution of A. actinomycetemcomitans serotypes between periodontal health and disease and point to possible variation in serotype determination due to bacterial growth and preparation procedures.

引用

页码：115 / 118

页数：4

共 20 条

[1] Alaluusua S, Asikainen S, Detection and distribution of Actinobacillus actinomycetemcomitans in the primary dentition, J Periodontol, 59, pp. 504-507, (1988)
[2] Asikainen S, Jousimies-Somer H, Kanervo A, Suramanen P, Certain bacterial species and morphotypes in localized juvenile periodontitis and matched controls, J Periodontol, 68, pp. 224-229, (1987)
[3] Asikainen S, Alaluusua S, Kleemola-Ku-jala E
[4] Baer PN, The case for periodontosis as a clinical entity, J Periodontol, 42, pp. 516-520, (1971)
[5] Campell DH, Garney JS, Cremer NE, Sussdorf DH, Nessler reaction, Methods in immunology, pp. 69-73, (1970)
[6] Chung H-J, Chung C-P, Son S-H, Nisengard RJ, Actinobacillus actinomycetemcomitans serotypes and leukotoxicity in Korean localized juvenile periodontitis, J Periodontol, 60, pp. 509-511, (1989)
[7] DiRienzo JM, Slots J, Genetic approach to the study of epidemiology and pathogenesis of Actinobacillus actinomycetemcomitans in localized juvenile periodontitis, Archives of Oral Biology, 35, pp. 79S-84S, (1990)
[8] Genco RJ, Zambon JJ, Christersson LA, Use and interpretation of microbiological assays in periodontal diseases, Oral Microbiol Immunol, 1, pp. 73-79, (1986)
[9] Gmur R, Hrodek K, Saxer UP, Guggenheim B, Double‐blind analysis of the relation between adult periodontitis and systemic host response to suspected periodontal pathogens, Infect Immun, 52, pp. 768-776, (1986)
[10] Hamada S, Masuda N, Ooshima T, Sobue S, Kotani S, Epidemiological survey of Streptococcus mutans among Japanese children: identification and serological typing of the isolated strains, Jpn J Microbiol, 20, pp. 33-44, (1976)

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