DETERMINATION OF META-CHLOROPHENYL-PIPERAZINE IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COULOMETRIC DETECTION

m-Chlorophenylpiperazine (mCPP) and the internal standard, 1-(2-pyrisidinyl) piperazine (IPP) are extracted from base matrix using CN sorbent extraction columns. Chromatography and detection are performed using isocratic reverse-phase high performance liquid chromatography (HPLC) with a CN column and coulometric end-point detection. The standard curve was linear over the range 0-25 ng/ml of plasma. The lower limit of quantitation is 0.2 ng/ml of mCPP from 1ml of plasma. The reproducibility (CV) of the method over the range of the standard curve varied from 3-8.5%. The inter-assay CV was 5.9%. Recovery averaged 92.5 +/- 8.6%. Plasma profiles of mCPP following varying doses of nefazodone HCl are given.