HETERODUPLEX FORMATION - A POTENTIAL SOURCE OF GENOTYPING ERROR FROM PCR PRODUCTS

被引：9

作者：

HATCHER, SLS

LAMBERT, QT

TEPLITZ, RL

CARLSON, JR

机构：

[1] University of California, Davis Medical Center, Diagnostic Molecular Pathology Laboratory, Department of Pathology, Sacramento, California

来源：

PRENATAL DIAGNOSIS | 1993年 / 13卷 / 03期

关键词：

HETERODUPLEX FORMATION; POLYMERASE CHAIN REACTION; GENOTYPING ERRORS; BETA-THALASSEMIA; PRENATAL DIAGNOSIS;

D O I：

10.1002/pd.1970130304

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The formation of heteroduplexes from polymerase chain reaction (PCR) products has recently become a diagnostic tool that is routinely used for the prenatal detection of small deletions or insertions in a number of disease-causing alleles. We present evidence illustrating that heterozygous PCR products can manifest 'invisible' heteroduplexes that can ultimately lead to genotyping errors. Justifications for these 'invisible' heteroduplexes and requisite parameters to optimize their detection are presented.

引用

页码：171 / 177

页数：7