DELTA-AMINOLEVULINATE UPTAKE BY RHIZOBIUM BACTEROIDS AND ITS LIMITATION BY THE PERIBACTEROID MEMBRANE IN LEGUME NODULES

Heme is overproduced during Rhizobium-Legume symbiosis and σ-aminolevulinate (ALA) is a common precursor in both bacterial and plant synthesis pathways of this molecule. ALA uptake by bacteroids from French bean and soybean nodules was characterized. The action of several metabolic inhibitors and the competition effect of malate on this uptake were studied. ALA transport appeared to be mediated by the dicarboxylate carrier system. Purified symbiosomes - bacteroids surrounded by the peribacteroid membrane - failed to accumulate significant amount of ALA. These experiments rule out the possibility for the plant cytosol to provide the bacteroid with ALA and strengthen the restrictive role of the peribacteroid membrane for exchanges between the two symbiotic partners. © 1992 Academic Press, Inc.