EXTRACELLULAR ANNEXIN-VI EXPRESSION IS ASSOCIATED WITH DIVALENT CATION-DEPENDENT ENDOTHELIAL-CELL ADHESION OF METASTATIC RAW117 LARGE-CELL LYMPHOMA-CELLS

We previously found that cell surface molecules of similar to 70, similar to 35, similar to 32, similar to 22, and similar to 14 kDa from liver-metastatic murine RAW117 large-cell lymphoma cells bound to target liver microvessel endothelial cells. Isolation and sequencing of the similar to 35-kDa component revealed it to be annexin II, a Ca2+-binding molecule involved in cytoskeletal and membrane interactions. Annexin II antibodies inhibited the adhesion of RAW117 tumor cells to live or fixed liver endothelial cells, and purified tumor cell surface fractions containing the similar to 35-kDa component inhibited partially RAW117 cell-endothelial cell adhesion, suggesting a role for annexins in tumor cell-endothelial cell adhesion. In the present study we identified the 70-kDa cell surface component that binds to hepatic sinusoidal endothelial cells in a Ca2+-dependent manner as annexin VI. Cytofluorographic analysis indicated that annexin VI was expressed on the cell surface in slightly higher amounts on highly metastatic RAW117 cells, and it was not removable by EDTA treatment. Anti-annexin VI antibodies inhibited the adhesion of RAW117 cells to fixed or unfixed murine hepatic sinusoidal endothelial cells by similar to 40%, indicating a role for annexin VI in mediating a portion of the Ca2+-dependent RAW117 cell adhesion to target liver microvessel endothelial cells. (C) 1994 Academic Press,Inc.