Processing of eukaryotic pre-rRNA: The role of the transcribed spacers

被引:57
作者
vanNues, RW [1 ]
Venema, J [1 ]
Rientjes, JMJ [1 ]
DirksMulder, A [1 ]
Raue, HA [1 ]
机构
[1] VRIJE UNIV AMSTERDAM,BIOCENTRUM AMSTERDAM,IMBW,DEPT BIOCHEM & MOLEC BIOL,1081 HV AMSTERDAM,NETHERLANDS
来源
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1995年 / 73卷 / 11-12期
关键词
ribosome; processing; precursor rRNA; eukaryote; transcribed spacer;
D O I
10.1139/o95-087
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The 17-18S, 5.8S, and 25-28S rRNA species of eukaryotic cells are produced by a series of nucleolytic reactions that liberate the mature rRNAs from the large primary precursor transcript synthesized by RNA polymerase I. Whereas the order of the cleavage reactions has long been established, until recently little information was available on their molecular details, such as the nature of the proteins, including the nucleolytic enzymes, involved and the signals directing the processing machinery to the correct sites. This situation is now rapidly changing, in particular where yeast is concerned. The use of recently developed systems for in vivo mutational analysis of yeast rDNA has considerably enhanced our knowledge of cis-acting structural features within the pre-rRNA, in particular the transcribed spacer sequences, that are critical for correct and efficient removal of these spacers. The same systems also allow a link to be forged between trans-acting processing factors and these cis-acting elements. In this paper, we will focus predominantly on the nature and role of the cis-acting processing elements as identified in the transcribed spacer regions of Saccharomyces cerevisiae pre-rRNA.
引用
收藏
页码:789 / 801
页数:13
相关论文
共 73 条
[1]  
BELTRAME M, 1994, NUCLEIC ACIDS RES, V22, P5139, DOI 10.1093/nar/22.23.5139
[2]   IDENTIFICATION AND FUNCTIONAL-ANALYSIS OF 2 U3 BINDING-SITES ON YEAST PRERIBOSOMAL RNA [J].
BELTRAME, M ;
TOLLERVEY, D .
EMBO JOURNAL, 1992, 11 (04) :1531-1542
[3]   LOCATION OF THE INITIAL CLEAVAGE SITES IN MOUSE PRE-RRNA [J].
BOWMAN, LH ;
GOLDMAN, WE ;
GOLDBERG, GI ;
HEBERT, MB ;
SCHLESSINGER, D .
MOLECULAR AND CELLULAR BIOLOGY, 1983, 3 (08) :1501-1510
[4]   MUTATIONS IN EUKARYOTIC 18S RIBOSOMAL-RNA AFFECT TRANSLATIONAL FIDELITY AND RESISTANCE TO AMINOGLYCOSIDE ANTIBIOTICS [J].
CHERNOFF, YO ;
VINCENT, A ;
LIEBMAN, SW .
EMBO JOURNAL, 1994, 13 (04) :906-913
[5]   THE RNA OF RNASE MRP IS REQUIRED FOR NORMAL PROCESSING OF RIBOSOMAL-RNA [J].
CHU, S ;
ARCHER, RH ;
ZENGEL, JM ;
LINDAHL, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (02) :659-663
[6]   NUCLEOTIDE-SEQUENCE DETERMINING THE 1ST CLEAVAGE SITE IN THE PROCESSING OF MOUSE PRECURSOR RIBOSOMAL-RNA [J].
CRAIG, N ;
KASS, S ;
SOLLNERWEBB, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (03) :629-633
[7]   SEQUENCE ORGANIZATION AND RNA STRUCTURAL MOTIFS DIRECTING THE MOUSE PRIMARY RIBOSOMAL-RNA-PROCESSING EVENT [J].
CRAIG, N ;
KASS, S ;
SOLLNERWEBB, B .
MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (01) :458-467
[8]  
DEJONGE P, 1977, EUR J BIOCHEM, V72, P361
[9]   SEPARATE PATHWAYS FOR EXCISION AND PROCESSING OF 16S AND 23S RIBOSOMAL-RNA FROM THE PRIMARY RIBOSOMAL-RNA OPERON TRANSCRIPT FROM THE HYPERTHERMOPHILIC ARCHAEBACTERIUM SULFOLOBUS-ACIDOCALDARIUS - SIMILARITIES TO EUKARYOTIC RIBOSOMAL-RNA PROCESSING [J].
DUROVIC, P ;
DENNIS, PP .
MOLECULAR MICROBIOLOGY, 1994, 13 (02) :229-242
[10]  
EICHLER DC, 1994, PROG NUCLEIC ACID RE, V49, P197