HANDLING OF OXALATE BY RAT-KIDNEY

被引:50
作者
GREGER, R
LANG, F
OBERLEITHNER, H
DEETJEN, P
机构
[1] Institut für Physiologie und Balneologie der Universität Innsbruck, Innsbruck, A-6010
来源
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1978年 / 374卷 / 03期
关键词
Microinfusion; Microperfusion; Organic acid secretion; Oxalate; Wistar rat;
D O I
10.1007/BF00585601
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Renal transport of14C-oxalate was studied in the rat by clearance and micropuncture techniques. The ultrafilterability of oxalate was 0.98±0.02 (n=7). Fractional clearance of oxalate was significantly above unity in antidiuresis and volume expansion: mean 1.24 ±0.04 (n=115). Pyrazinamide (1.1·10-3 mol/kg BW) and probenecid (0.35·10-3 mol/kg BW) had no significant effect on oxalate clearance. P-aminohippurate (1.45·10-3 mol/kg BW) and urate (0.48 ·10-3 mol/kg BW) depressed the fractional clearance of oxalate significantly from 116 to 91 and from 125 to 90%, respectively. Excess excretion of14C-oxalate over3H-inulin was invariably demonstrable in peritubular microperfusion experiments (n=5) and in microinfusions underneath the kidney capsule (n=4). Together with the first 50% of3H-inulin 58±2% of the total14C-oxalate were excreted in the peritubular microperfusions, and 64±3% in the subcapsular microinfusions. In tubular microinfusion experiments (n=36) urinary14C-oxalate recovery was almost complete after early proximal microinfusion (93±4%) and complete after late proximal microinfusion (102±4%). In continuous microperfusion experiments of proximal tubules (n=42) a small but highly significant outflux of14C-oxalate of 7% per mm perfusion distance was found. The data suggest that oxalate is freely filterable at the glomerular site. A small but significant amount of oxalate is reabsorbed in the proximal nephron. Most likely at the same site and in the pars recta oxalate is secreted and tubular load increased to 124% of filtered load. This amount is excreted in final urine. The secretion of oxalate is inhibited by organic acids which are known to be secreted by the proximal tubule and the pars recta. © 1978 Springer-Verlag.
引用
收藏
页码:243 / 248
页数:6
相关论文
共 25 条
[1]   COLORIMETRIC DETERMINATION OF URINARY OXALATE RECOVERED AS CALCIUM-OXALATE - APPLICATION OF A SIMPLE CORRECTION FACTOR FOR INCOMPLETE PRECIPITATION [J].
BAADENHUIJSEN, H ;
JANSEN, AP .
CLINICA CHIMICA ACTA, 1975, 62 (02) :315-324
[2]   ON METABOLISM OF L-ASCORBIC ACID IN SCORBUTIC GUINEA-PIG [J].
BANAY, M ;
DIMANT, E .
BIOCHIMICA ET BIOPHYSICA ACTA, 1962, 59 (02) :313-&
[3]  
BAUMANN K, 1967, PFLUG ARCH GES PHYS, V294, pR21
[4]   EFFECT OF INHIBITORS OF TRANSTUBULAR TRANSPORT ON RENAL EXCRETION OF URIC ACID IN RAT [J].
BOUDRY, JF .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1971, 328 (04) :279-&
[5]   ZUR BIOCHEMIE DER OXALSAURE .1. SCHICKSAL UND HALBWERTSZEIT IM ORGANISMUS DER RATTE ABBAU DURCH ASPERGILLUS-NIGER [J].
BRUBACHER, G ;
JUST, M ;
BODUR, H ;
BERNHARD, K .
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1956, 304 (2-4) :173-181
[6]  
CATTELL WR, 1962, CLIN SCI, V22, P43
[7]  
COSTELLO J, 1976, J LAB CLIN MED, V87, P903
[8]   DER TUBULARE TRANSPORT VON P-AMINOHIPPURSAURE - MIKROPERFUSIONSVERSUCHE AM EINZELNEPHRON DER RATTENNIERE IN SITU [J].
DEETJEN, P ;
SONNENBE.H .
PFLUGERS ARCHIV FUR DIE GESAMTE PHYSIOLOGIE DES MENSCHEN UND DER TIERE, 1965, 285 (01) :35-&
[9]   EFFECT OF SALINE INFUSION ON SODIUM REABSORPTION BY PROXIMAL TUBULE OF DOG [J].
DIRKS, JH ;
CIRKSENA, WJ ;
BERLINDE.RW .
JOURNAL OF CLINICAL INVESTIGATION, 1965, 44 (07) :1160-&
[10]   SITE OF RENAL PHOSPHATE REABSORPTION - MICROPUNCTURE AND MICROINFUSION STUDY [J].
GREGER, R ;
LANG, F ;
MARCHAND, G ;
KNOX, FG .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1977, 369 (02) :111-118