KERATINS AS MARKERS THAT DISTINGUISH NORMAL AND TUMOR-DERIVED MAMMARY EPITHELIAL-CELLS

被引：182

作者：

TRASK, DK ^{[1
]}

BAND, V ^{[1
]}

ZAJCHOWSKI, DA ^{[1
]}

YASWEN, P ^{[1
]}

SUH, T ^{[1
]}

SAGER, R ^{[1
]}

机构：

[1] HARVARD UNIV, SCH MED,DANA FARBER CANC INST,DIV CANC GENET, 44 BINNEY ST, BOSTON, MA 02115 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 06期

关键词：

Breast cancer; Immortalization; Intermediate filaments; Tumor and normal antigens;

D O I：

10.1073/pnas.87.6.2319

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Keratin 5 (K5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. To extend these findings, the full complements of keratins in normal, immortalized, and tumor cells were compared. It is shown here that normal cells produce keratins K5, K6, K7, K14, and K17, whereas tumor cells produce mainly keratins K8, K18, and K19. In immortalized cells, which are preneoplastic or partially transformed, the levels of KS mRNA and protein are lower than in normal cells, whereas the amount of K18 is increased. Thus, K5 is an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased K5 expression correlates with tumorigenic progression.

引用

页码：2319 / 2323

页数：5

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