BACTERIAL CONJUGATION BETWEEN ESCHERICHIA-COLI AND PSEUDOMONAS SPP - DONOR AND RECIPIENT CELLS IN SOIL

被引:10
作者
BERG, G [1 ]
TREVORS, JT [1 ]
机构
[1] UNIV GUELPH,DEPT ENVIRONM BIOL,GUELPH N1G 2W1,ONTARIO,CANADA
来源
JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1990年 / 5卷 / 2-3期
关键词
Bacteria; Conjugation; Gene transfer; Plasmids; Soil; Survival;
D O I
10.1007/BF01573856
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Experiments conducted in microcosms containing loam soil samples inoculated with either E. coli or Pseudomonas spp. donor and recipient cells showed that bacterial cells survived and conjugated over a 24-h incubation period. E. coli transconjugants were detected 6 h after donor and recipient strains were introduced into sterile soil samples. In non-sterile soil samples, transconjugants were detected between 8 and 24 h incubation. Pseudomonas transconjugants were recovered from sterile soil samples between 6 and 12 h after their introduction and as early as 2 h in non-sterile soil. The results show that genetic interactions occur in non-sterile soil in relatively short periods of time at relatively high transfer frequencies (10-3 to 10-4). Studies on genetic interactions in soil are becoming necessary in risk assessment/environmental impact studies prior to the release of genetically engineered or modified organisms into uncontained environments. © 1990 Society for Industrial Microbiology.
引用
收藏
页码:79 / 84
页数:6
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