DETERMINATION OF TRIMETHOPRIM IN BIOLOGICAL-FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：34

作者：

WEINFELD, RE

MACASIEB, TC

机构：

[1] Department of Pharmacokinetics and Biopharmaceutics, Hoffmann-La Roche Inc., Nutley

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 164卷 / 01期

关键词：

D O I：

10.1016/S0378-4347(00)81573-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid, sensitive, and specific high-performance liquid chromatographic assay was developed for the determination of trimethoprim in blood, plasma, and urine using normal-phase (adsorption) chromatography on a microparticulate silica column and UV monitoring at 280 nm. Trimethoprim is selectively extracted from the biological sample matrix at alkaline pH with chloroform, providing nearly quantitative extraction (>95%) and a sensitivity limit of 0.01 to 0.02 μg/ml blood or plasma, without interference from sulfonamides. © 1979.

引用

页码：73 / 84

页数：12

共 15 条

[1] DETERMINATION OF TRIMETHOPRIM AND ITS N-OXIDE METABOLITES IN URINE OF MAN, DOG, AND RAT BY DIFFERENTIAL PULSE POLAROGRAPHY
BROOKS, MA
DESILVA, JAF
DARCONTE, L
[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 1973, 62 (08) : 1395 - 1397
[2] BROOKS MA, 1973, ANAL CHEM, V45, P263
[3] TRIMETHOPRIM A SULPHONAMIDE POTENTIATOR
BUSHBY, SRM
HITCHING.GH
[J]. BRITISH JOURNAL OF PHARMACOLOGY, 1968, 33 (01) : 72 - +
[4] ANALYTICAL APPROACH TO QUANTITATION OF KNOWN DRUGS IN HUMAN BIOLOGICAL SAMPLES BY HPLC
BYE, A
BROWN, ME
[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1977, 15 (09) : 365 - 371
[5] FLOREY K, 1978, ANAL PROFILES DRUG S, V7, P453
[6] GRUNBERG E, 1966, ANTIMICROB AG CHEMOT, V1965, P430
[7] TRIMETHOPRIM AND SULPHAMETHOXAZOLE IN TREATMENT OF CHRONIC CHEST INFECTIONS
HUGHES, DTD
DREW, CDM
JOHNSON, TBW
JARVIS, JD
[J]. CHEMOTHERAPY, 1969, 14 (03) : 151 - &
[8] SEMIAUTOMATED SPECTROPHOTOFLUOROMETRIC DETERMINATION OF TRIMETHOPRIM IN BIOLOGICAL-FLUIDS
KAPLAN, SA
WEINFELD, RE
LEE, TL
[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 1973, 62 (11) : 1865 - 1870
[9] PHARMACOKINETIC PROFILE OF TRIMETHOPRIM-SULFAMETHOXAZOLE IN MAN
KAPLAN, SA
WEINFELD, RE
ABRUZZO, CW
MCFADEN, K
JACK, ML
WEISSMAN, L
[J]. JOURNAL OF INFECTIOUS DISEASES, 1973, 128 : S547 - S555
[10] SIMULTANEOUS DETERMINATION OF VANCOMYCIN, ANISOMYCIN, AND TRIMETHOPRIM LACTATE BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY
KIRCHMEIER, RL
UPTON, RP
[J]. ANALYTICAL CHEMISTRY, 1978, 50 (02) : 349 - 351

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