BETA-ADRENERGIC REGULATION OF BETA-ACTIN MESSENGER-RNA ABUNDANCE IN MOUSE PAROTID-GLANDS BY A POSTTRANSCRIPTIONAL MECHANISM

被引:12
作者
ROBERTS, SGE [1 ]
COPE, GH [1 ]
MCDONALD, CJ [1 ]
机构
[1] UNIV SHEFFIELD,DEPT BIOMED SCI,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND
关键词
D O I
10.1677/jme.0.0060079
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In the first 24 h after a single injection of the beta-adrenergic agonist isoprenaline to mice, the level of beta-actin mRNA in the parotid glands increased significantly above that observed in untreated mice. The increase was transient, reaching 11 times the normal level 18 h after treatment and declining thereafter. Repeated daily doses of isoprenaline did not result in any further increase in beta-actin mRNA. Nuclear transcription experiments showed that there was no increase in the transcription rate of the beta-actin gene 8 h after an injection of isoprenaline, although beta-actin mRNA levels were increasing at this time. Immunoblotting revealed an increase in beta-actin protein in parotid gland samples after isoprenaline treatment, although the increase was not to the same extent as the mRNA, perhaps indicating that degradation of beta-actin had also increased. Using immunocytochemistry it was found that beta-actin was located mainly in the apical cortex of the normal acinar cell. There was a significant decrease in cortical beta-actin 24 h after isoprenaline treatment, suggesting that the beta-actin was under the process of redistribution. From these data we propose that isoprenaline caused an increase in beta-actin synthesis by a post-transcriptional mechanism and a redistribution of beta-actin in preparation for the well-known subsequent change in morphology and function of the parotid glands.
引用
收藏
页码:79 / 86
页数:8
相关论文
共 39 条
[1]   BASIC PROLINE-RICH PROTEINS OF MURINE PAROTID-GLANDS - INDUCTION OF MESSENGER-RNA BY ISOPRENALINE AND POST-SECRETION PROCESSING [J].
BANNISTER, AJ ;
DIVECHA, N ;
ASHMORE, M ;
MCDONALD, CJ .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 181 (02) :371-379
[2]  
BASERGA R, 1970, FED PROC, V29, P1443
[3]   THE POLY(A)-POLY(A)-BINDING PROTEIN COMPLEX IS A MAJOR DETERMINANT OF MESSENGER-RNA STABILITY INVITRO [J].
BERNSTEIN, P ;
PELTZ, SW ;
ROSS, J .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (02) :659-670
[4]   POLY(A), POLY(A) BINDING-PROTEIN AND THE REGULATION OF MESSENGER-RNA STABILITY [J].
BERNSTEIN, P ;
ROSS, J .
TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (09) :373-377
[5]   DETERMINANTS OF MESSENGER-RNA STABILITY [J].
BRAWERMAN, G .
CELL, 1987, 48 (01) :5-6
[6]   REGULATION OF C-MYC MESSENGER-RNA STABILITY INVITRO BY A LABILE DESTABILIZER WITH AN ESSENTIAL NUCLEIC-ACID COMPONENT [J].
BREWER, G ;
ROSS, J .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (05) :1996-2006
[7]   ENLARGEMENT OF SALIVARY GLAND IN MICE TREATED WITH ISOPROPYLNORADRENALINE [J].
BROWNGRANT, K .
NATURE, 1961, 191 (479) :1076-&
[8]   IRON-RESPONSIVE ELEMENTS - REGULATORY RNA SEQUENCES THAT CONTROL MESSENGER-RNA LEVELS AND TRANSLATION [J].
CASEY, JL ;
HENTZE, MW ;
KOELLER, DM ;
CAUGHMAN, SW ;
ROUAULT, TA ;
KLAUSNER, RD ;
HARFORD, JB .
SCIENCE, 1988, 240 (4854) :924-928
[9]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[10]   A POTENTIAL ROLE FOR RNA TRANSCRIBED FROM B2 REPEATS IN THE REGULATION OF MESSENGER-RNA STABILITY [J].
CLEMENS, MJ .
CELL, 1987, 49 (02) :157-158