SEPARATION OF THE COMPLETE THIOREDOXIN PATTERN OF SOYBEAN LEAVES (GLYCINE-MAX) BY HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY ON MONO-Q

被引：9

作者：

HABERLEIN, I

机构：

[1] Fachbereich Biologie-Chemie der Universität Kassel, W-3500 Kassel

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 587卷 / 01期

关键词：

D O I：

10.1016/0021-9673(91)85204-S

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Chromatographic studies are essential for assessing the physiological roles of the multiple thioredoxins (TRs) found in plants. Green leaves of the soybean (Glycine max) were shown to contain six different thioredoxins, which could be separated by anion-exchange chromatography. Three different thioredoxin activities were obtained by DEAE-cellulose chromatography (TRI, TRII and TRIII), which were further resolved by high-performance anion-exchange chromatography on a Mono Q column into TRIa, TRIb, TRIIa, TRIIb and TRIIc; TRIII could not be subdivided into additional thioredoxin species. In isolated chloroplasts of soybean leaves only TRIa and TRIb have been detected.

引用

页码：109 / 115

页数：7

共 20 条

[1]

BERTSERMANN A, 1983, EUR J BIOCHEM, V131, P339

[2] ANIMAL AND PLANT-MITOCHONDRIA CONTAIN SPECIFIC THIOREDOXINS [J].