DISSECTION OF THE METHYL-CPG BINDING DOMAIN FROM THE CHROMOSOMAL PROTEIN MECP2

被引：466

作者：

NAN, XS ^{[1
]}

MEEHAN, RR ^{[1
]}

BIRD, A ^{[1
]}

机构：

[1] UNIV EDINBURGH,INST CELL & MOLEC BIOL,KINGS BLDG,MAYFIELD RD,EDINBURGH EH9 3JR,MIDLOTHIAN,SCOTLAND

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 21期

基金：

英国惠康基金;

关键词：

D O I：

10.1093/nar/21.21.4886

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MeCP2 is a chromosomal protein which binds to DNA that is methylated at CpG. In situ immunofluorescence in mouse cells has shown that the protein is most concentrated in pericentromeric heterochromatin, suggesting that MeCP2 may play a role in the formation of inert chromatin. Here we have isolated a minimal methyl-CpG binding domain (MBD) from MeCP2. MBD is 85 amino acids in length, and binds exclusively to DNA that contains one or more symmetrically methylated CpGs. MBD has negligable non-specific affinity for DNA, confirming that non-specific and methyl-CpG specific binding domains of MeCP2 are distinct. In vitro footprinting indicates that MBD binding can protect a 12 nucleotide region surrounding a methyl-CpG pair, with an approximate dissociation constant of 10(-9)M.

引用

页码：4886 / 4892

页数：7

共 24 条

[1] SPECIFIC PROTECTION OF METHYLATED CPGS IN MAMMALIAN NUCLEI [J].