TFC3 - GENE ENCODING THE B-BLOCK BINDING SUBUNIT OF THE YEAST TRANSCRIPTION FACTOR-IIIC

被引：57

作者：

LEFEBVRE, O

CARLES, C

CONESA, C

SWANSON, RN

BOUET, F

RIVA, M

SENTENAC, A

机构：

[1] CTR ETUD SACLAY, DEPT BIOL CELLULAIRE & MOLEC, SERV BIOCHIM & GENET MOLEC, F-91191 GIF SUR YVETTE, FRANCE

[2] CTR ETUD SACLAY, DEPT ENGN & ETUD PROT, F-91191 GIF SUR YVETTE, FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 21期

关键词：

RNA POLYMERASE-III; TRANSFER RNA; INTRON; EPITOPE TAGGING;

D O I：

10.1073/pnas.89.21.10512

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Yeast transcription factor IIIC (TFIIIC) is a multisubunit protein complex that interacts with two control elements of class III promoters called the A and B blocks. Here we describe the gene encoding the 138-kDa subunit (tau138), which is involved in B-block binding. From the DNA sequence, the open reading frame, interrupted by an intron with an unusual 3' splice acceptor site, is in agreement with all the microsequencing data for peptides within tau138. TFC3 is a single-copy gene located on chromosome I; it is essential for cell viability as shown by a gene disruption experiment. Epitope-tagging of the TFC3 gene product and DNA binding experiments are consistent with the presence of one copy of tau138 in TFIIIC-DNA complexes.

引用

页码：10512 / 10516

页数：5

共 30 条

[1] 2 COMPONENTS OF SACCHAROMYCES-CEREVISIAE TRANSCRIPTION FACTOR-IIIB (TFIIIB) ARE STEREOSPECIFICALLY LOCATED UPSTREAM OF A TRANSFER-RNA GENE AND INTERACT WITH THE 2ND-LARGEST SUBUNIT OF TFIIIC [J].