CHARACTERIZATION OF THE 5'-FLANKING REGION OF THE HEPATOCYTE GROWTH-FACTOR GENE

被引:14
作者
ARAVAMUDAN, B
WATABE, M
WATABE, K
机构
[1] Department of Medical Microbiology, Southern Illinois University School of Medicine, Springfield, IL 62702
关键词
D O I
10.1006/bbrc.1993.2050
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The hepatocyte growth factor is the most potent mitogen for hepatocytes in primary culture and is involved in liver regeneration. The expression of the gene appears to be tightly controlled by various humoral factors. To understand the molecular mechanism of the gene expression, we cloned and determined the nucleotide sequence of the 5’-flanking region of the gene. In this region, there are sequences homologous to responding elements of P53, Rb, IL−1, IL−6, glucocorticoids, TPA and TGF-β. We also identified three major transcriptional initiation sites by primer extension analysis of this region. Functional analyses of this region by constructing CAT reporter plasmids indicate that the sequence functions in a tissue specific manner and there is a negative regulatory region which suppresses the gene expression in rat transformed kidney cells. © 1993 Academic Press, Inc.
引用
收藏
页码:346 / 353
页数:8
相关论文
共 27 条
[1]   12-O-TETRADECANOYL-PHORBOL-13-ACETATE INDUCTION OF THE HUMAN COLLAGENASE GENE IS MEDIATED BY AN INDUCIBLE ENHANCER ELEMENT LOCATED IN THE 5'-FLANKING REGION [J].
ANGEL, P ;
BAUMANN, I ;
STEIN, B ;
DELIUS, H ;
RAHMSDORF, HJ ;
HERRLICH, P .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (06) :2256-2266
[3]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[4]  
DIRENZO MF, 1992, ONCOGENE, V7, P2549
[5]   GROWTH-HORMONE PROMOTES EARLY INITIATION OF HEPATOCYTE GROWTH-FACTOR GENE-EXPRESSION IN THE LIVER OF HYPOPHYSECTOMIZED RATS AFTER PARTIAL-HEPATECTOMY [J].
EKBERG, S ;
LUTHER, M ;
NAKAMURA, T ;
JANSSON, JO .
JOURNAL OF ENDOCRINOLOGY, 1992, 135 (01) :59-67
[6]  
FURLONG RA, 1991, J CELL SCI, V100, P173
[7]   TRANSFER OF MOTOGENIC AND INVASIVE RESPONSE TO SCATTER FACTOR HEPATOCYTE GROWTH-FACTOR BY TRANSFECTION OF HUMAN MET PROTOONCOGENE [J].
GIORDANO, S ;
ZHEN, Z ;
MEDICO, E ;
GAUDINO, G ;
GALIMI, F ;
COMOGLIO, PM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (02) :649-653
[8]   PURIFICATION AND PARTIAL CHARACTERIZATION OF HEPATOCYTE GROWTH-FACTOR FROM PLASMA OF A PATIENT WITH FULMINANT HEPATIC-FAILURE [J].
GOHDA, E ;
TSUBOUCHI, H ;
NAKAYAMA, H ;
HIRONO, S ;
SAKIYAMA, O ;
TAKAHASHI, K ;
MIYAZAKI, H ;
HASHIMOTO, S ;
DAIKUHARA, Y .
JOURNAL OF CLINICAL INVESTIGATION, 1988, 81 (02) :414-419
[9]   RECOMBINANT GENOMES WHICH EXPRESS CHLORAMPHENICOL ACETYLTRANSFERASE IN MAMMALIAN-CELLS [J].
GORMAN, CM ;
MOFFAT, LF ;
HOWARD, BH .
MOLECULAR AND CELLULAR BIOLOGY, 1982, 2 (09) :1044-1051
[10]   RAPID AND MARKED INDUCTION OF HEPATOCYTE GROWTH-FACTOR DURING LIVER-REGENERATION AFTER ISCHEMIC OR CRUSH INJURY [J].
HAMANOUE, M ;
KAWAIDA, K ;
TAKAO, S ;
SHIMAZU, H ;
NOJI, S ;
MATSUMOTO, K ;
NAKAMURA, T .
HEPATOLOGY, 1992, 16 (06) :1485-1492