A METHOD FOR IN-VIVO DIFFUSION TRACER STUDIES COMBINING PERFUSION FIXATION WITH INTRAVENOUS TRACER INJECTION

A method permitting exact time studies in diffusion tracer experiments in vivo is described. The method consist in application of perfusion fixation via the aorta abdominalis after the injection of the tracer substance. As the animal can be prepared for perfusion before the tracer is injected, time accuracy is in the order of a few seconds. Some applications of the method are demonstrated. A further advantage of the method is good tissue preservation. This perfusion technique is also recommended for study when exact timing is necessary, for example in autoradiography. © 1969 Springer-Verlag.