ELECTRON-MICROSCOPIC INDIA INK TECHNIQUE FOR DEMONSTRATING CAPSULES ON MICROORGANISMS - STUDIES WITH STREPTOCOCCUS-PNEUMONIAE, STAPHYLOCOCCUS-AUREUS, AND NEISSERIA-GONORRHOEAE

被引:21
作者
MELLY, MA
MCGEE, ZA
HORN, RG
MORRIS, F
GLICK, AD
机构
[1] VANDERBILT UNIV,SCH MED,DEPT MED,DIV INFECT DIS,NASHVILLE,TN 37240
[2] VANDERBILT UNIV,SCH MED,DEPT PATHOL,NASHVILLE,TN 37240
关键词
D O I
10.1093/infdis/140.4.605
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A technique using India ink in electron microscopic preparations was used to study bacterial capsules. Capsules were demonstrated on Streptococcus pneumoniae and Staphylococcus aureus (strain M and the Smith diffuse variant) from in vitro cultures. Two types of false capsules were observed on Neisseria gonorrhoeae grown in vitro or obtained from human urethral exudates. No true capsules were definitively identified on gonococci from these sources. The technique described does not require the use of specific anticapsular antibody or specific polysaccharide stains and permits the detection of artifacts that may, by the use of light microscopic methods, be misinterpreted as capsules. © 1979 by The University of Chicago.
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页码:605 / 609
页数:5
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