GROWTH-INHIBITORY EFFECTS OF 1,25-DIHYDROXYVITAMIN-D3 ON NORMAL HUMAN KERATINOCYTES CULTURED IN SERUM-FREE MEDIUM

被引：118

作者：

MATSUMOTO, K

HASHIMOTO, K

NISHIDA, Y

HASHIRO, M

YOSHIKAWA, K

机构：

[1] Department of Dermatology, Osaka University School of Medicine, Fukushima, Fukushima-ku, Osaka

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90898-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] on the growth of normal human keratinocytes cultured in serum-free medium was investigated. 1,25(OH)2D3 inhibited the cell growth at 10-7 M by 75.3% and at 10-6 M almost completely. The growth inhibition was accompanied by changes related to proliferation: (1) remarkable inhibition of DNA synthesis, (2) the decrease in the number of high-affinity receptors for epidermal growth factor, with almost no change in total receptor number, (3) the rapid decrease in c-myc mRNA level. The inhibition of DNA synthesis and the decrease of c-myc mRNA expression occurred at 3 h after the addition of 1,25(OH)2D3. These results suggest that decrease of c-myc mRNA expression is one of the primary effects of 1,25(OH)2D3 in the growth inhibition of human keratinocytes. © 1990.

引用

页码：916 / 923

页数：8

共 28 条

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