FLOW-COULOMETRIC DETECTOR FOR URIC-ACID IN HUMAN URINE

被引：14

作者：

UCHIYAMA, S ^{[1
]}

UMESATO, F ^{[1
]}

SUZUKI, S ^{[1
]}

SATO, T ^{[1
]}

机构：

[1] OSAKA GAS CO LTD,RES CTR,KONOHANA KU,OSAKA 554,JAPAN

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 230卷 / 01期

关键词：

D O I：

10.1016/S0003-2670(00)82781-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel flow-coulometric detector integrating an immobilized uricase reactor and an electrolytic cell was fabricated and used for the determination of uric acid in human urine. The procedure is based on the measurement of the total charge with and without passing the sample through an enzyme reactor which allows the complete conversion of the electro-active uric acid in electro-inactive products. The amount of uric acid is linearly related to the difference between the two total charges. The current efficiencies for 1 × 10-4-1 × 10-3 M uric acid were found to be nearly 100% (r.s.d. < 1%). © 1990.

引用

页码：195 / 198

页数：4

共 11 条

[1] DERIVATIZATION OF CROSS-LINKED POLYACRYLAMIDE BEADS . CONTROLLED INTRODUCTION OF FUNCTIONAL GROUPS FOR PREPARATION OF SPECIAL-PURPOSE, BIOCHEMICAL ADSORBENTS [J].