MOLECULAR-WEIGHTS FROM APPROACH-TO-SEDIMENTATION EQUILIBRIUM DATA USING NON-LINEAR REGRESSION-ANALYSIS

Data obtained from early times during the transient period of sedimentation equilibrium experiments are analyzed using an approximate solution to the Lamm equation to estimate s/D. The Cr versus r data obtained at several times during approach-to-equilibrium are analyzed using a nonlinear least squares algorithm and Fujita's approximate solution. This procedure was tested using D-Ser13-somatostatin, ribonuclease, and ovalbumin. The results obtained demonstrate that for monodisperse samples s/D may be rapidly and reliably estimated using this method. © 1979.