EXPRESSION OF ALPHA(4) INTEGRIN MESSENGER-RNA AND PROTEIN AND FIBRONECTIN IN THE EARLY CHICKEN-EMBRYO

被引:49
作者
STEPP, MA
URRY, LA
HYNES, RO
机构
[1] MIT,HOWARD HUGHES MED INST,DEPT BIOL,CAMBRIDGE,MA 02139
[2] GEORGE WASHINGTON UNIV,MED CTR,DEPT ANAT,WASHINGTON,DC 20037
关键词
INTEGRINS; EARLY EMBRYO; NEURAL CREST; SOMITES;
D O I
10.3109/15419069409014210
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
alpha(4) integrins (alpha(4) beta(1) and alpha(4) beta(7)) have been shown to mediate both cell-matrix adhesion to fibronectin and cell-cell adhesion to VCAM-1. These interactions have been suggested to contribute to hematopoiesis, lymphocyte homing, recruitment of inflammatory cells, neural crest cell migration and myogenesis. We report here the cloning of chicken alpha(4) cDNA and its use to define the patterns of expression of alpha(4) mRNA and protein in early chicken embryos (19-22 somite pairs), a stage at which neural crest cells can be examined at various points in their migration and semitic development and differentiation can also be observed at various stages. We observe widespread expression of both alpha(4) mRNA and protein, although the patterns of steady state expression do not conform precisely. Many neural crest cells contain significant levels of alpha(4) mRNA. Some neural crest cells express alpha(4) protein but its expression is transient and/or limited to a subset of these cells. alpha(4) is strongly expressed at both mRNA and protein levels by semitic cells and their derivatives in the sclerotome, dermatome and myotome and is also expressed in neural tube, otic placode, heart, gut endoderm and some other tissues. Comparison with the distributions of fibronectin shows that, although some alpha(4) expression occurs in locations consistent with a role in cell-matrix adhesion to fibronectin, alpha(4) is also expressed in other places where fibronectin is low or absent and a role for alpha(4) in cell-cell interactions appears more likely.
引用
收藏
页码:359 / 375
页数:17
相关论文
共 57 条
[1]  
ABO T, 1981, J IMMUNOL, V127, P1024
[2]  
ADAMS JC, 1993, DEVELOPMENT, V117, P1183
[3]   INTEGRINS AND OTHER CELL-ADHESION MOLECULES [J].
ALBELDA, SM ;
BUCK, CA .
FASEB JOURNAL, 1990, 4 (11) :2868-2880
[4]  
BEDNARCZYK JL, 1992, CLIN EXP METASTAS, V10, P281
[5]   CHARACTERIZATION OF THE INTEGRIN ALPHA-8 SUBUNIT - A NEW INTEGRIN BETA-1-ASSOCIATED SUBUNIT, WHICH IS PROMINENTLY EXPRESSED ON AXONS AND ON CELLS IN CONTACT WITH BASAL LAMINAE IN CHICK-EMBRYOS [J].
BOSSY, B ;
BOSSYWETZEL, E ;
REICHARDT, LF .
EMBO JOURNAL, 1991, 10 (09) :2375-2385
[6]  
BRONNERFRASER M, 1992, DEVELOPMENT, V115, P197
[7]   ANALYSIS OF THE EARLY STAGES OF TRUNK NEURAL CREST MIGRATION IN AVIAN EMBRYOS USING MONOCLONAL-ANTIBODY HNK-1 [J].
BRONNERFRASER, M .
DEVELOPMENTAL BIOLOGY, 1986, 115 (01) :44-55
[8]  
CHAN BMC, 1992, J BIOL CHEM, V267, P8366
[9]  
CHAN BMC, 1991, J IMMUNOL, V147, P398
[10]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299