TRANSCRIPTIONAL ACTIVITIES OF A WINGED BEAN KUNITZ CHYMOTRYPSIN INHIBITOR GENE PROMOTER IN STABLE AND TRANSIENT EXPRESSION SYSTEMS

被引：8

作者：

SAKATA, Y ^{[1
]}

FUKUSHIMA, H ^{[1
]}

HABU, Y ^{[1
]}

FURUYA, S ^{[1
]}

NAITO, S ^{[1
]}

OHNO, T ^{[1
]}

机构：

[1] MINIST AGR FORESTRY & FISHERIES, TROP AGR RES CTR, OKINAWA BRANCH, ISHIGAKI 907, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 11期

关键词：

D O I：

10.1271/bbb.58.2104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequential deletions of the promoter region of the WCI-3b gene, which encodes the major chymotrypsin inhibitor of winged bean, were constructed and their expression was analyzed in transgenic tobacco plants and in bombarded winged bean seeds. In transgenic tobacco plants, a critical promoter region which is important for high levels of expression in seeds was identified, but deletion of this region had essentially no effect when bombarded into winged bean seeds.

引用

页码：2104 / 2106

页数：3

共 20 条

[1] PHOTOREGULATION OF A PHYTOCHROME GENE PROMOTER FROM OAT TRANSFERRED INTO RICE BY PARTICLE BOMBARDMENT
BRUCE, WB
CHRISTENSEN, AH
KLEIN, T
FROMM, M
QUAIL, PH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (24) : 9692 - 9696
[2] POSITIVE AND NEGATIVE CIS-ACTING DNA DOMAINS ARE REQUIRED FOR SPATIAL AND TEMPORAL REGULATION OF GENE-EXPRESSION BY A SEED STORAGE PROTEIN PROMOTER
BUSTOS, MM
BEGUM, D
KALKAN, FA
BATTRAW, MJ
HALL, TC
[J]. EMBO JOURNAL, 1991, 10 (06) : 1469 - 1479
[3] A TRANS-ACTING FACTOR THAT BINDS TO A GT-MOTIF IN A PHYTOCHROME GENE PROMOTER
DEHESH, K
BRUCE, WB
QUAIL, PH
[J]. SCIENCE, 1990, 250 (4986) : 1397 - 1399
[4] FROMM M, 1987, METHOD ENZYMOL, V153, P351
[5] FUJIWARA T, 1991, PLANT CELL REP, V9, P602, DOI 10.1007/BF00231797
[6] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS
GAMBORG, OL
MILLER, RA
OJIMA, K
[J]. EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) : 151 - +
[7] IDENTIFICATION OF FUNCTIONAL DOMAINS IN THE MAIZE TRANSCRIPTIONAL ACTIVATOR C1 - COMPARISON OF WILD-TYPE AND DOMINANT INHIBITOR PROTEINS
GOFF, SA
CONE, KC
FROMM, ME
[J]. GENES & DEVELOPMENT, 1991, 5 (02) : 298 - 309
[8] NUCLEAR FACTORS BINDING TO THE EXTENSIN PROMOTER EXHIBIT DIFFERENTIAL ACTIVITY IN CARROT PROTOPLASTS AND CELLS
GRANELL, A
PERETO, JG
SCHINDLER, U
CASHMORE, AR
[J]. PLANT MOLECULAR BIOLOGY, 1992, 18 (04) : 739 - 748
[9] STRUCTURE AND REGULATED EXPRESSION OF KUNITZ CHYMOTRYPSIN INHIBITOR GENES IN WINGED BEAN (PSOPHOCARPUS-TETRAGONOLOBUS (L) DC)
HABU, Y
PEYACHOKNAGUL, S
UMEMOTO, K
SAKATA, Y
OHNO, T
[J]. JOURNAL OF BIOCHEMISTRY, 1992, 111 (02) : 249 - 258
[10] JEFFERSON RA, 1987, EMBO J, V6, P3901

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