TRYPANOSOMA-CONGOLENSE - DEVELOPMENTAL REGULATION OF PROTEIN-KINASES AND TYROSINE PHOSPHORYLATION DURING THE LIFE-CYCLE

被引:14
作者
PARSONS, M
CARTER, V
MUTHIANI, A
MURPHY, N
机构
[1] UNIV WASHINGTON, DEPT PATHOBIOL, SEATTLE, WA 98195 USA
[2] INT LABS RES ANIM DIS, NAIROBI, KENYA
关键词
DEVELOPMENT; PROTEIN SERINE THREONINE KINASE; TYROSINE PHOSPHORYLATION; PVDF; POLYVINYLIDENE DIFLUORIDE; SDS-PAGE; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS;
D O I
10.1006/expr.1995.1063
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
In higher eukaryotes, key steps in the control of growth and proliferation are regulated by protein phosphorylation. However, little is known about the role of protein phosphorylation in the developmental cycles of pathogenic protozoa. In Trypanosoma brucei, only the bloodform and procyclic form stages can be obtained in sufficient numbers for biochemical analyses. However, the entire life cycle of Trypanosoma congolense can be generated in vitro, providing sufficient material for analyses of the different developmental stages. The studies reported here provide a series of snapshots documenting the activity of a number of protein serine/threonine kinases and the pattern of tyrosine-phosphorylated proteins throughout the T. congolense developmental cycle. Metacyclic forms and mammalian bloodforms showed similar profiles of protein kinase activity, as did procyclic forms and epimastigotes. Most tyrosine-phosphorylated proteins were shared between all developmental stages, with the exception of a 100-kDa metacyclic-specific species. The developmental changes in molecules involved in protein phosphorylation in the different developmental stages support the concept that changes in protein phosphorylation networks are important correlates of the developmental process in African trypanosomes. (C) 1995 Academic Press, Inc.
引用
收藏
页码:507 / 514
页数:8
相关论文
共 18 条
[1]   PHOSPHORYLATION DIFFERENCES AMONG PROTEINS OF BLOOD-STREAM DEVELOPMENTAL STAGES OF TRYPANOSOMA-BRUCEI-BRUCEI [J].
ABOAGYEKWARTENG, T ;
OLEMOIYOI, OK ;
LONSDALEECCLES, JD .
BIOCHEMICAL JOURNAL, 1991, 275 :7-14
[2]   TRYPANOSOMA (NANNOMONAS) CONGOLENSE - CHANGES IN RESPIRATORY METABOLISM DURING THE LIFE-CYCLE [J].
BIENEN, EJ ;
WEBSTER, P ;
FISH, WR .
EXPERIMENTAL PARASITOLOGY, 1991, 73 (04) :403-412
[3]  
COOPER JA, 1983, METHOD ENZYMOL, V99, P387
[4]   STAGE-SPECIFIC REGULATION OF PROTEIN-PHOSPHORYLATION IN LEISHMANIA-MAJOR [J].
DELL, KR ;
ENGEL, JN .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1994, 64 (02) :283-292
[5]   SIGNALING BY RECEPTOR TYROSINE KINASES [J].
FANTL, WJ ;
JOHNSON, DE ;
WILLIAMS, LT .
ANNUAL REVIEW OF BIOCHEMISTRY, 1993, 62 :453-481
[6]  
FERRELL JE, 1989, J BIOL CHEM, V264, P20723
[7]  
FISH WR, 1991, BIOCHEMISTRY-US, V28, P5415
[8]  
GALE M, 1994, J CELL SCI, V107, P1825
[9]  
HANKS SK, 1991, METHOD ENZYMOL, V200, P38
[10]   DIFFERENTIAL ACCUMULATION OF A PROTEIN-KINASE HOMOLOG IN TRYPANOSOMA-BRUCEI [J].
HUA, SB ;
WANG, CC .
JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, 54 (01) :20-31