A SIMPLE SPECTROPHOTOMETRIC SCANNING PROCEDURE FOR QUANTITATIVE ESTIMATION OF PROTEIN IN POLYACRYLAMIDE GELS

被引:14
作者
DRAVID, AR
FREDEN, H
LARSSON, S
机构
[1] Institute of Neurobiology, Faculty of Medicine, University of Göteborg, Göteborg
来源
JOURNAL OF CHROMATOGRAPHY | 1969年 / 41卷 / 01期
关键词
D O I
10.1016/0021-9673(64)80097-2
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple and rapid spectrophotometric procedure for the scanning of polyacrylamide gels in ultraviolet light is described. It is shown that the absorption of the albumin band in acrylamide gel is proportional to the quantity of albumin subjected to electrophoresis and that the procedure can be applied to the study of serum and tissue protein separations in polyacrylamide gels. Some of the advantages of this method over other currently used methods are indicated. © 1969.
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页码:53 / &
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