DERIVATION OF CLONES FROM THE CHOROIDEREMIA LOCUS BY PREPARATIVE FIELD INVERSION GEL-ELECTROPHORESIS

被引：20

作者：

VANDEPOL, TJR ^{[1
]}

CREMERS, FPM ^{[1
]}

BROHET, RM ^{[1
]}

WIERINGA, B ^{[1
]}

ROPERS, HH ^{[1
]}

机构：

[1] CATHOLIC UNIV NIJMEGEN, RADBOUD HOSP, DEPT HUMAN GENET, POB 9101, 6500 HB NIJMEGEN, NETHERLANDS

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 04期

关键词：

D O I：

10.1093/nar/18.4.725

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By making use of preparative field inversion gel electrophoresis, we have constructed a lambdaZAP library that is highly enriched for sequences from the chorolderemia locus. In vivo excision of pBluescript SK(-) constructs from lambdaZAP obviates the subcloning of DNA inserts and allows for rapid processIng of several hundred recombinants. From 625 kb Sf/1 fragment we isolated 7 clones that were physically mapped using microdeletlons associated with the disease. One of these clones is located within, or Just telomeric to, the chorolderemia gene and detects two restriction fragment length polymorphisms (RFLPs). Another clone detects a RFLP which maps centromeric to the disease locus. Together these probes should Improve the reliability of linkage analysis in choroideremia families and should pave the way for the Isolation of the choroideremia gene. © 1990 Oxford University Press.

引用

页码：725 / 731

页数：7

共 40 条

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