SEPARATION OF THE CYTOCHROMES-P-450 IN PIG-KIDNEY MITOCHONDRIA CATALYZING-1-ALPHA-HYDROXYLATIONS, 24-HYDROXYLATIONS AND 26-HYDROXYLATIONS OF 25-HYDROXYVITAMIN-D3

被引：15

作者：

POSTLIND, H

机构：

[1] Department of Pharmaceutical Biochemistry, University of Uppsala, S-751 23 Uppsala

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91702-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytochromes P-450 in pig kidney mitochondria catalyzing 1α-, 24- and 26-hydroxylations of 25-hydroxyvitamin D3 have been separated. The cytochrome P-450 fractions required NADPH, mitochondrial ferredoxin and ferredoxin reductase for catalytic activity. The present report demonstrates that different forms of cytochrome P-450 are involved in 1α-, 24- and 26-hydroxylations of 25-hydroxyvitamin D3 and provides a basis for further purification and characterization of these enzymes. © 1990.

引用

页码：261 / 266

页数：6

共 16 条

[1]

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[2] 25-HYDROXYLATION OF VITAMIN-D3 BY A CYTOCHROME-P-450 FROM RABBIT LIVER-MITOCHONDRIA [J].