GROWTH OF THE WW STRAIN OF THEILER VIRUS IN MOUSE CENTRAL NERVOUS-SYSTEM ORGANOTYPIC CULTURE

被引：20

作者：

WROBLEWSKA, Z

KIM, SU

SHEFFIELD, WD

GILDEN, DH

机构：

[1] UNIV PENN,DEPT NEUROL,PHILADELPHIA,PA 19104

[2] UNIV PENN,DEPT PATHOL,DIV NEUROPATHOL,PHILADELPHIA,PA 19104

来源：

ACTA NEUROPATHOLOGICA | 1979年 / 47卷 / 01期

关键词：

Mouse central nervous system culture; Theiler virus;

D O I：

10.1007/BF00698267

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Myelinated organotypic cultures of mouse central nervous system (CNS) provide a favorable milieu for growth of the WW strain of Theiler virus (TV), WW-TV induced a cytopathic effect characterized by neuronal destruction and swelling of myelin sheaths. Tissue culture medium and homogenates of infected cultures produced neurologic disease in mice, and TV. was demonstrated in cultures by indirect immunoflourescence and electron microscopy. Ultrastructurally, severe alterations in neurons and the presence of cytoplasmic inclusions containing paracrystalline arrays of 30-nm virus particles were evident in astrocytes. Demyelination appeared to be secondary to lysis of oligodendrocytes by virus. CNS organotypic cultures provide an environment for the study of TV-induced demyelination. © 1979 Springer-Verlag.

引用

页码：13 / 19

页数：7

共 15 条

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[2]

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