SUBSTITUTIONS OF TRYPTOPHAN RESIDUES IN HUMAN CORTICOSTEROID-BINDING GLOBULIN - IMPACT ON STEROID-BINDING AND GLYCOSYLATION

被引:18
作者
AVVAKUMOV, GV
HAMMOND, GL
机构
[1] UNIV WESTERN ONTARIO,DEPT OBSTET & GYNAECOL,LONDON,ON,CANADA
[2] UNIV WESTERN ONTARIO,DEPT BIOCHEM,LONDON,ON,CANADA
[3] UNIV WESTERN ONTARIO,DEPT ONCOL,LONDON,ON,CANADA
[4] UNIV WESTERN ONTARIO,MRC,FETAL & NEONATAL HLTH & DEV GRP,LONDON,ON,CANADA
基金
英国医学研究理事会;
关键词
D O I
10.1016/0960-0760(94)90010-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human corticosteroid-binding globulin (CBG) contains four tryptophan residues at positions 141, 185, 266 and 371; one of which is thought to be located in the steroid-binding site. These residues were substituted by site-directed mutagenesis and expression of mutant CBG cDNAs in Chinese hamster ovary cells. Analyses of the resulting mutants indicate that Trp(371) is most likely located in the steroid-binding site, and that hydrophobic interactions between Trp(141) and the steroid molecule or other amino-acids in the CBG polypeptide may also contribute to high-affinity interactions between CBG and its steroid ligands. In addition, substitution of Trp(266) resulted in altered glycosylation of CBG, and this supports the concept that it participates in intra-molecular carbohydrate-polypeptide interactions which may influence the conformation and secretion of this glycoprotein.
引用
收藏
页码:191 / 194
页数:4
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