OVGA1BP, A FILARIAL ANTIGEN WITH HOMOLOGY TO VERTEBRATE GALACTOSIDE-BINDING PROTEINS

被引:52
作者
KLION, AD
DONELSON, JE
机构
[1] UNIV IOWA HOSP & CLIN,DEPT BIOCHEM,IOWA CITY,IA 52242
[2] UNIV IOWA HOSP & CLIN,HOWARD HUGHES MED INST,IOWA CITY,IA 52242
关键词
FILARIASIS; LOA LOA; ONCHOCERCA VOLVULUS; GALACTOSIDE-BINDING PROTEIN;
D O I
10.1016/0166-6851(94)90081-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The increased incidence of ''allergic'' symptomatology and clinical complications seen in non-endemic individuals with loiasis, as compared to natives of endemic areas, is thought to reflect a heightened immune response to filarial antigens. To identify antigens involved in this hyperresponsiveness, a cDNA library constructed from adult female RNA from the related filarial parasite, Onchocerca volvulus, was screened with serum from a North American who acquired loiasis in West Africa. Sequence analysis of one of the identified clones, OvGalBP, revealed significant homology to the vertebrate S-type lectins, a family of thiol-dependent, metal-independent galactoside binding lectins, which includes an IgE-binding protein thought to be involved,in IgE regulation. The 1100-bp insert of OvcalBP contains the entire protein coding region and has a 3' poly(A) tail. The two amino acid consensus sequences (WGxExR and HFNPRF) found in all of the S-type lectins are present. Purified recombinant protein expressed as a fusion with glutathione-S-transferase (OVGalBP-GST) was recognized by sera from a majority of filaria-infected patients but not by putatively immune individuals from an endemic area or by unexposed endemic and non-endemic controls. Interestingly, OvGalBP-GST specifically bound IgE (and not IgG) in a lactose-inhibitable manner, suggesting a potential role for this protein in the pathophysiology of human filarial infection.
引用
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页码:305 / 315
页数:11
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