AN UPDATING OF THE BIOCHEMICAL FUNCTION OF COENZYME-Q IN MITOCHONDRIA

被引：29

作者：

LENAZ, G ^{[1
]}

FATO, R ^{[1
]}

CASTELLUCCIO, C ^{[1
]}

CAVAZZONI, M ^{[1
]}

ESTORNELL, E ^{[1
]}

HUERTAS, JF ^{[1
]}

PALLOTTI, F ^{[1
]}

CASTELLI, GP ^{[1
]}

RAUCHOVA, H ^{[1
]}

机构：

[1] UNIV BOLOGNA, DIPARTIMENTO BIOCHIM GIOVANNI MORUZZI, I-40126 BOLOGNA, ITALY

来源：

MOLECULAR ASPECTS OF MEDICINE | 1994年 / 15卷

关键词：

D O I：

10.1016/0098-2997(94)90010-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The apparent K-m for coenzyme Q(10) in NADH oxidation by coenzyme Q (CoQ) -extracted beef heart mitochondria is close to their CoQ content, whereas both succinate and glycerol-3-phosphate oxidation (the latter measured in hamster brown adipose tissue mitochondria) are almost saturated at physiological CoQ concentration. Attempts to enhance NADH oxidation rate by excess CoQ incorporation in vitro were only partially successful: the reason is in the limited amount of CoQ(10) that can be incorporated in monomeric form, as shown by lack of fluorescence quenching of membrane fluorescent probes; at difference with CoQ(10), CoQ(5) quenches probe fluorescence and likewise enhances NADH oxidation rate above normal. Attempts to enhance the CoQ content in perfused rat liver and in isolated hepatocytes failed to show uptake in the purified mitochondrial fraction. Nevertheless CoQ cellular uptake is able to protect mitochondrial activities. Incubation of hepatocytes with adriamycin induces loss of respiration and mitochondrial potential measured in whole cells by flow cytometry using rhodamine 123 as a probe: concomitant incubation with CoQ(10) completely protects both respiration and potential. An experimental study of aging in the rat has shown some decrease of mitochondrial CoQ content in heart, and less in liver and skeletal muscle. In spite of the little change observed, it is reasoned that CoQ administration may be beneficial in the elderly, owing to the increased demand for antioxidants.

引用

页码：29 / 36

页数：8

共 18 条

[1]

BEYER RE, 1987, CHEM SCRIPTA, V27, P145

[2] THE DISCOVERY OF COENZYME-Q - A COMMENTARY ON ISOLATION OF A QUINONE FROM BEEF-HEART MITOCHONDRIA [J].

CRANE, FL .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1000 :358-363

[3]

CRANE FL, 1993, CLIN INVESTIGATOR, V71, pS55

[4] PEROXIDE-PRODUCING POTENTIAL OF TISSUES - INVERSE CORRELATION WITH LONGEVITY OF MAMMALIAN-SPECIES [J].

CUTLER, RG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (14) :4798-4802

[5]

DARZYNKIEWICZ Z, 1982, CANCER RES, V42, P799

[6] UBIQUINOL - AN ENDOGENOUS ANTIOXIDANT IN AEROBIC ORGANISMS [J].

ERNSTER, L ;

FORSMARKANDREE, P .

CLINICAL INVESTIGATOR, 1993, 71 (08) :S60-S65

[7] INCORPORATION OF UBIQUINONE HOMOLOGS INTO LIPID VESICLES AND MITOCHONDRIAL-MEMBRANES [J].

ESPOSTI, MD ;

BERTOLI, E ;

PARENTICASTELLI, G ;

FATO, R ;

MASCARELLO, S ;

LENAZ, G .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1981, 210 (01) :21-32

[8] SATURATION KINETICS OF COENZYME-Q IN NADH AND SUCCINATE OXIDATION IN BEEF-HEART MITOCHONDRIA [J].

ESTORNELL, E ;

FATO, R ;

CASTELLUCCIO, C ;

CAVAZZONI, M ;

CASTELLI, GP ;

LENAZ, G .

FEBS LETTERS, 1992, 311 (02) :107-109

[9] DETERMINATION OF PARTITION AND LATERAL DIFFUSION-COEFFICIENTS OF UBIQUINONES BY FLUORESCENCE QUENCHING OF NORMAL-(9-ANTHROYLOXY)STEARIC ACIDS IN PHOSPHOLIPID-VESICLES AND MITOCHONDRIAL-MEMBRANES [J].

FATO, R ;

BATTINO, M ;

ESPOSTI, MD ;

CASTELLI, GP ;

LENAZ, G .

BIOCHEMISTRY, 1986, 25 (11) :3378-3390

[10]

GENOVA ML, 1995, MOL ASPECTS MED S, V15, pS47

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