INCREASED CAPACITY FOR STORE REGULATED CALCIUM INFLUX IN U937 CELLS DIFFERENTIATED BY TREATMENT WITH DIBUTYRYL-CAMP

被引：10

作者：

DAVIS, W

HALLIWELL, EL

SAGE, SO

ALLEN, JM

机构：

[1] UNIV GLASGOW,DIV BIOCHEM & MOLEC BIOL,IBLS,GLASGOW G12 8QQ,LANARK,SCOTLAND

[2] UNIV GLASGOW,DEPT MED & THERAPEUT,GLASGOW,LANARK,SCOTLAND

[3] UNIV CAMBRIDGE,PHYSIOL LAB,CAMBRIDGE,ENGLAND

来源：

CELL CALCIUM | 1995年 / 17卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/0143-4160(95)90108-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have investigated the mechanisms underlying calcium signalling evoked by cross-linking of the high affinity IgG receptor (Fc gamma RI) in populations of the human monocyte-like cell line, U937, following activation of the cells by cytokine treatment, or differentiation to a more macrophage-like state by treatment with dibutyryl cAMP (Bt(2)cAMP). We have shown previously that a larger and more prolonged entry of external calcium occurs in Bt(2)cAMP-differentiated cells, although there is a smaller initial release from internal stores in these cells than in those activated by IFN-gamma treatment. In this paper we demonstrate, by use of the endomembrane Ca2+-ATPase inhibitor, thapsigargin, that this effect is explained (at least in part) by an enhanced capacity for store regulated entry of calcium in Bt(2)cAMP-differentiated cells.

引用

页码：345 / 353

页数：9

共 24 条

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