PRESENCE OF AMP-RICH RNA IN COUNTER-CURRENT DISTRIBUTION AND COLUMN CHROMATOGRAPHY FRACTIONS OF PEA ROOT NUCLEIC ACIDS

Nucleic acids differentially extracted from pea roots with 4-aminosalicylate were subjected to counter-current distribution. The RNA traveling farthest in the mobile, organic phase contained 49 % AMP. When nucleic acids in this fraction were subjected to column chromatography on methylated albumin-kieselguhr, much of the RNA was tenaciously bound to the column. If column chromatography preceded counter-current distribution, only part of the tenaciously bound RNA was rich in AMP and moved with the organic phase. The base composition of the AMP-rich fraction was about the same whether all the differentially extracted nucleic acids or only the tenaciously bound nucleic acids were taken for counter-current distribution. © 1968.