98QUANTITATIVE ANALYSIS OF SULFOLIPID (SULFOQUINOVOSYL DIGLYCERIDE] AND GALACTOLIPIDS (MONOGALACTOSYL AND DIGALACTOSYL DIGLYCERIDES] IN PLANT TISSUES

被引：328

作者：

ROUGHAN, PG

BATT, RD

机构：

[1] Plant Physiology Division, D. S. I. R., Palmerston North

[2] Department of Chemistry and Biochemistry, Massey University, Palmerston North

来源：

ANALYTICAL BIOCHEMISTRY | 1968年 / 22卷 / 01期

关键词：

D O I：

10.1016/0003-2697(68)90261-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for the quantitative and rapid estimation of sulfolipid and galactolipids in plant tissues. The procedure is based on the isolation of pure glycolipids by a combination of DEAE-cellulose column and thin-layer chromatography. The sugar component is estimated by the reaction of the lipid on the thin-layer adsorbent with phenol and sulfuric acid. Alternatively, the lipid is deacylated on the adsorbent in 2 N sulfuric acid and an aliquot of the hydrolyzate is reacted with phenol and sulfuric acid. Very small amounts of sulfo- and galacto-lipids can be reproducibly estimated by this method. © 1968.

引用

页码：74 / &

共 39 条

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