HORMONAL REQUIREMENTS FOR THE INDUCTION OF CYTOCHROME-P450 IN HEPATOCYTES CULTURED IN A SERUM-FREE MEDIUM

被引：81

作者：

SINCLAIR, JF

SINCLAIR, PR

BONKOWSKY, HL

机构：

[1] DARTMOUTH COLL,SCH MED,DEPT MED,HANOVER,NH 03755

[2] DARTMOUTH COLL,SCH MED,DEPT BIOCHEM,HANOVER,NH 03755

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(79)91771-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Drug mediated induction of cytochrome P450 was studied in cultures of hepatocytes that had never been cultured in the presence of serum. Propylisopropylacetamide induced a five-fold increase in cytochrome P450, approximating in ovo induced levels, when triiodothyronine and/or dexamethasone were included in the culture medium. Insulin was apparently not required for this induction. Cytochrome P450, free of cytochrome oxidase, could be fully recovered from cell homogenates in a 8700g supernatant, by use of a buffer containing 0.2% Emulgen. © 1979.

引用

页码：710 / 717

页数：8

共 19 条

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