BIOASSAY OF INTERLEUKIN-1 IN SERUM AND PLASMA FOLLOWING REMOVAL OF INHIBITORY ACTIVITY WITH POLYETHYLENE-GLYCOL

被引：28

作者：

HOPKINS, SJ

HUMPHREYS, M

机构：

[1] University of Manchester Rheumatic Diseases Centre, Hope Hospital, Salford, M6 8HD, Clinical Sciences Building, Eccles Old Rd.

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 133卷 / 01期

关键词：

Bioassay; Inhibitor; Interleukin-1; Polyethylene glycol;

D O I：

10.1016/0022-1759(90)90326-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Serum and plasma normally inhibit the responsiveness of the indicator cells used in bioassays for interleukin-1 (IL-1). A precipitation step with 12% polyethylene glycol (PEG) was found to remove material responsible for such inhibition. The IL-1 bioassay could be performed in the presence of a 3% concentration of PEG and recovery of added natural IL-1 from plasma or serum was essentially complete. © 1990.

引用

页码：127 / 131

页数：5

共 11 条

[1]

CANNON JG, 1988, LYMPHOKINE RES, V7, P457

[2]

CHILDS C, 1990, IN PRESS PATHOPHYSIO

[3] PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION FROM COMPLEMENTARY-DNA OF A HUMAN INTERLEUKIN-1 RECEPTOR ANTAGONIST [J].

EISENBERG, SP ;

EVANS, RJ ;

AREND, WP ;

VERDERBER, E ;

BREWER, MT ;

HANNUM, CH ;

THOMPSON, RC .

NATURE, 1990, 343 (6256) :341-346

[4] SIMPLE, SENSITIVE AND SPECIFIC BIOASSAY OF INTERLEUKIN-1 [J].

HOPKINS, SJ ;

HUMPHREYS, M .

JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 120 (02) :271-276

[5]

HOPKINS SJ, 1988, CLIN EXP IMMUNOL, V72, P422

[6]

HOPKINS SJ, 1990, IN PRESS PATHOPHYSIO

[7] SENSITIVE INVITRO BIOASSAY FOR THE MEASUREMENT OF SERUM FOLLICLE-STIMULATING-HORMONE [J].

JIA, XC ;

HSUEH, AJW .

NEUROENDOCRINOLOGY, 1985, 41 (05) :445-448

[8]

NEWTON RC, 1988, LYMPHOKINE RES, V7, P207

[9]

SECKINGER P, 1987, J IMMUNOL, V139, P1546

[10] RAPID, SENSITIVE DETECTION OF PLASMA IL-1 BY EXTRACTION AND BIOASSAY [J].

SERIES, JJ ;

SHAPIRO, D ;

CAMERON, I ;

SMITH, J ;

FRASER, WD ;

GARDEN, OJ ;

SHENKIN, A .

JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 108 (1-2) :33-37

← 1 2 →