DEVELOPMENT OF AN OPTIMIZED SYSTEM FOR ELECTROPORATION OF LISTERIA SPECIES

被引：17

作者：

ALEXANDER, JE ^{[1
]}

ANDREW, PW ^{[1
]}

JONES, D ^{[1
]}

ROBERTS, IS ^{[1
]}

机构：

[1] UNIV LEICESTER,DEPT MICROBIOL,MED SCI BLDG,UNIV RD,LEICESTER LE1 9HN,ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1990年 / 10卷 / 04期

关键词：

D O I：

10.1111/j.1472-765X.1990.tb00109.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Electroporation was used to facilitate transformation of Listeria species with plasmid DNA. Optimal conditions for transformation of L. monocytogenes were a field strength of 8·5 kV/cm, 200 Ohms resistance, 25 μF capacitor with a time constant of 5 ms. With these conditions, 3·9 × 106 transformants/pg DNA were obtained. Under the same conditions, L. innocua and L. iuanouii exhibited a frequency of transformation similar to that of L. monocytogenes but a somewhat lower level was obtained with L. seeligeri. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：179 / 181

页数：3

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