DETERMINATION OF THE QUATERNARY COMPOUND CICLOTROPIUM IN HUMAN BIOLOGICAL-MATERIAL AFTER HYDROLYSIS AND DERIVATIZATION WITH THE FLUOROPHOR FLUNOXAPROFEN CHLORIDE

被引：5

作者：

LIEBMANN, B ^{[1
]}

HENKE, D ^{[1
]}

SPAHNLANGGUTH, H ^{[1
]}

MUTSCHLER, E ^{[1
]}

机构：

[1] UNIV FRANKFURT,PHARMAKOL INST NAT WISSENSCH,THEODOR STERN KAI 7,GEBAUDE 75A,W-6000 FRANKFURT 1,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 572卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80482-R

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The quantitative determination of the quaternary spasmolytic compound ciclotropium and its metabolite N-isopropyltropinium is described for human plasma and urine. The analytical procedure consists of ion-pair extraction from biological material, alkaline hydrolysis, subsequent derivatization with the fluorophor flunoxaprofen chloride and separation by high-performance liquid chromatography on a reversed-phase column with fluorimetric monitoring. The detection limits of 0.5 ng/ml in plasma and 10 ng/ml in urine at signal-to-noise ratios higher than 3 permit the determination of pharmacokinetic parameters after therapeutic doses.

引用

页码：181 / 193

页数：13

共 8 条

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