XH1 - A NEW CERVICAL-CARCINOMA CELL-LINE AND XENOGRAFT MODEL OF TUMOR INVASION, METASTASIS AND REGRESSION

被引:10
作者
HAN, X
LYLE, R
EUSTACE, DLS
JEWERS, RJ
PARRINGTON, JM
DAS, A
CHANA, T
DAGG, B
MONEY, S
BATES, TD
KENNEY, A
HEYDERMAN, E
机构
[1] UNITED MED & DENT SCH GUY & ST THOMAS HOSP,ST THOMAS HOSP,DEPT HISTOPATHOL,LONDON SE1 7EH,ENGLAND
[2] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,RICHARD DIMBLEBY LAB CANC VIROL,LONDON SE1 7EH,ENGLAND
[3] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,DEPT OBSTET & GYNAECOL,LONDON SE1 7EH,ENGLAND
[4] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,DEPT RADIOTHERAPY,LONDON SE1 7EH,ENGLAND
[5] UNIV LONDON UNIV COLL,MRC,HUMAN BIOCHEM GENET UNIT,LONDON NW1 2HE,ENGLAND
关键词
D O I
10.1038/bjc.1991.376
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
A new cell line, XH1, has been derived from an invasive focally keratinising adenosquamous carcinoma of the cervix in a 32 year old patient. It has been maintained in long term monolayer culture for 26 months, and passaged over 100 times (>> 300 population doublings). It is aneuploid with a mean chromosome number of 78. Examination using two minisatellite hypervariable DNA probes has shown it to be different from other cell lines maintained in this laboratory and from HeLa. Two sublines, XH1a and XH1b, show marked differences in monolayer culture, growth in soft agar, and xenograft formation. XH1 and XH1a cell readily form subcutaneous xenografts, and lung colonies can be established by their intravenous injection. Subcutaneous injection of XH1b cells results in rapid cell growth for a few days after which the tumour undergoes degeneration and then regresses completely. The XH1 karyotype has many rearranged chromosomes. Parental XH1 cells and both sublines show integration of HPV16 into the genome.
引用
收藏
页码:645 / 654
页数:10
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