VIABILITY OF HUMAN DIPLOID CELLS AS A FUNCTION OF IN-VITRO AGE

被引：166

作者：

MERZ, GS

ROSS, JD

机构：

[1] Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania

[2] Institute for Basic Research in Mental Retardation, Staten Island, New York

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 1969年 / 74卷 / 03期

关键词：

D O I：

10.1002/jcp.1040740302

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The fraction of cell capable of division was determined for (1) population of the human diploid cell strains, WI38 after different numbers of subcultivations in vitro and (2) a single population of WI38 cells at intervals throughout its entire in vitro lifespan. In both cases the percentage of cells capable of division decreased with increasing age in tissue culture. The rate and the magnitude of the decrease is sufficient to account for the limited in vitro lifespan reported by other investigators. Furthermore, the decrease in the fraction of cells capable of division in similar in some respects of senescence among human populations. Copyright © 1969 Wiley‐Liss, Inc.

引用

页码：219 / &