EVIDENCE FOR 2 STRUCTURAL GENES FOR ALKALINE-PHOSPHATASE IN BACILLUS-SUBTILIS

被引：79

作者：

HULETT, FM

BOOKSTEIN, C

JENSEN, K

机构：

[1] Lab. for Molecular Biology, University of Illinois, Chicago

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 02期

关键词：

D O I：

10.1128/jb.172.2.735-740.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two secreted alkaline phosphatase proteins were purified from cultures of Bacillus subtilis JH646MS. The two proteins showed slight differences in subunit molecular weight, substrate specificity, and charge characteristics. A total of 62% of the first 22 amino-terminal amino acids were identical. Both sequences showed conservation of structural features identified in Escherichia coli and human alkaline phosphatases. One alkaline phosphatase was a monomer and the other was a dimer. Southern analysis of genomic DNA with degenerative oligomers based on the amino acid sequences suggest that there are two structural genes for alkaline phosphatase in the genome of B. subtilis.

引用

页码：735 / 740

页数：6

共 23 条

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