SPIN-LABELED OLIGONUCLEOTIDES SITE SPECIFICALLY LABELED AT THE INTERNUCLEOTIDE LINKAGE - SEPARATION OF STEREOISOMERIC PROBES AND EPR SPECTROSCOPICAL DETECTION OF HYBRID FORMATION IN SOLUTION

被引：23

作者：

NAGAHARA, S

MURAKAMI, A

MAKINO, K

机构：

[1] Department of Polymer Science and Engineering, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606, Matsugasaki

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 1992年 / 11卷 / 2-4期

关键词：

D O I：

10.1080/07328319208021747

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Described herein is the synthesis, separation of stereoisomers, hybridization, and EPR behavior of spin-labeled oligonucleotides site specifically labeled at the internucleotide linkage with 4-amino-TEMPO. The results obtained indicate that the spin-labeling method will provide a DNA probe useful for detecting hybrid formation with the complementary segment in solution.

引用

页码：889 / 901

页数：13

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