ADCC-MEDIATING CAPACITY IN CHILDREN WITH COWS MILK PROTEIN INTOLERANCE IN RELATION TO IGG SUBCLASS PROFILE OF SERUM ANTIBODIES TO BETA-LACTOGLOBULIN

被引:9
作者
SAALMAN, R
DAHLGREN, UI
FALLSTROM, SP
HANSON, LA
WOLD, AE
AHLSTEDT, S
机构
[1] MOLNDAL CTY HOSP, DEPT PAEDIAT, MOLNDAL, SWEDEN
[2] PHARMACIA DIAGNOST AB, S-75182 UPPSALA, SWEDEN
关键词
D O I
10.1111/j.1365-3083.1995.tb03637.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In a previous study sera from children with cow's milk protein intolerance (CMPI) exhibiting gastrointestinal symptoms were found to efficiently induce antibody-dependent cell-mediated cytotoxicity (ADCC) to beta-lactoglobulin-coated cells. In contrast, sera from children with coeliac disease showed a low ADCC-mediating capacity, despite high levels of IgG anti-beta-lactoglobulin antibodies. The study described here was undertaken to evaluate whether differences in IgG subclass profile of anti-beta-lactoglobulin antibodies could explain the observed variations in the ADCC-mediating capacity. Forty-eight sera from the following groups of children were investigated: CMPI with predominantly gastrointestinal symptoms, CMPI with skin symptoms of immediate-onset, children with untreated coeliac disease and healthy references. Absorption experiments indicated that primarily IgG1 antibodies were responsible for the ADCC-mediating capacity of the sera. Accordingly, the ADCC reactivity of individual sera correlated with their IgG1 antibody levels. Sera from CMPI children with gastrointestinal symptoms, most of which had a high ADCC reactivity, also demonstrated a distinctive subclass pattern of their anti-beta-lactoglobulin antibodies with higher relative proportions of IgG1 (ratios: IgG1/IgG, IgG1/IgG3 and IgG1/IgG4) than those from the other diagnostic groups. Using logistic regression analysis, the diagnostic potential of ADCC as well as of different IgG subclass variables for the recognition of gastrointestinal symptoms caused by CMPI was evaluated. The ADCC reactivity of sera was found to be the best predictor in this model.
引用
收藏
页码:140 / 146
页数:7
相关论文
共 29 条
  • [1] Walker-Smith J, Diseases of the small intestine in childhood, pp. 144-184, (1988)
  • [2] Strobel S, Immunologically mediated damage to the intestinal mucosa, Acta Paediatr Scand Suppl, 365, pp. 46-57, (1990)
  • [3] van der Meulen FW, van der Hart M, Fleer A, von dem Borne AE, Engelfriet CP, van Loghem JJ, The role of adherence to human mononuciear phagocytes in the destruction of red cells sensitized with non‐complement binding IgG antibodies, Br J Haematol, 38, pp. 541-549, (1978)
  • [4] Shorter RG, McGill DB, Bahn RC, Cytotoxicity of mononuciear cells for autologous colonic epithelial cells in colonic diseases, Gastroenterology, 86, pp. 13-22, (1984)
  • [5] Das KM, Kadono Y, Fleischner GM, Antibody‐dependent cell‐mediated cytotoxicity in serum samples from patients with ulcerative colitis. Relationship to disease activity and response to total colectomy, Am J Med, 77, pp. 791-796, (1984)
  • [6] Saalman R, Carlsson B, Fallstrom SP, Hanson LA, Ahlstedt S, Antibody‐dependent cell‐mediated cytotoxicity to beta‐lactoglo‐bulin‐coated cells with sera from children with intolerance of cow's milk protein, Clin Exp Immunol, 85, pp. 446-452, (1991)
  • [7] Anderson CL, Looney RJ, Immunoglobulin G Fc receptors of human leukocytes, Methods Enzymol, 150, pp. 524-536, (1987)
  • [8] Ljunggren K, Broliden PA, Morfeldt-Manson L, Jondal M, Wahren B, IgG subclass response to HIV in relation to antibody‐dependent cellular cytotoxicity at different clinical stages, Clin Exp Immunol, 73, pp. 343-347, (1988)
  • [9] Wigzcll H, Antibody‐dependent cell‐mediated cytotoxicity, Antibody‐dependent cell‐mediated cytotoxicity, pp. 1-13, (1985)
  • [10] Wettergren B, Jodal U, Jonasson G, Epidemiology of bacteriuria dunng the first year of life, Acta Paediatr Scand, 74, pp. 925-933, (1985)