DETECTION OF RIBOSE-METHYLATED NUCLEOTIDES IN ENZYMATIC HYDROLYSATES OF RNA BY THERMOSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：17

作者：

TAKEDA, N

POMERANTZ, SC

MCCLOSKEY, JA

机构：

[1] UNIV UTAH,DEPT MED CHEM,SALT LAKE CITY,UT 84112

[2] UNIV UTAH,DEPT BIOCHEM,SALT LAKE CITY,UT 84112

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 562卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80580-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A procedure has been developed for the detection and characterization of ribose-methylated dinucleotides of the type NmpN' in enzymatic digests of RNA. Differences in reaction products from hydrolysis using RNase T2, which will not cleave NmpN', and hydrolysis by nuclease P1 are analyzed by thermospray liquid chromatography-mass spectrometry. The method is applicable to dinucleotides present in nanogram range quantities and is suited for the characterization of new or unexpected ribose-methylated nucleotides for which chromatographic mobilities are not known.

引用

页码：225 / 235

页数：11

共 21 条

[1] A NEW SOFT IONIZATION TECHNIQUE FOR MASS-SPECTROMETRY OF COMPLEX-MOLECULES [J].