SEPARATION OF LENS-CULINARIS HEMAGGLUTININ BY FORCED FLOW ELECTROPHORESIS

被引：5

作者：

BIER, M

LONG, T

RYU, HW

机构：

[1] center for separation science, university of arizona, tucson, arizona

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 1990年 / 25卷 / 9-10期

基金：

美国国家航空航天局;

关键词：

D O I：

10.1080/01496399008050380

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The usefulness and versatility of forced flow electrophoresis (FFE) for the separation of proteins were examined with Lens culinaris hemagglutinin (lentil lectins). FFE was operated both in the usual single stage and in a more effective, new double-stage scheme. The dependence of the resolution on the flow velocity through the permeable channel and on the electrical field were analyzed. Increased effectiveness in separation was obtained with various pretreatments such as electrofil-tration, dialysis, and centrifugation. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：997 / 1005

页数：9

共 21 条

[1]

BIER M, 1967, T AM SOC ART INT ORG, V13, P227

[2] NEW PRINCIPLE OF PREPARATIVE ELECTROPHORESIS [J].