USE OF WATER-SOLUBLE 1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)CARBODIIMIDE FOR THE FLUORESCENT DETERMINATION OF URONIC-ACIDS AND CARBOXYLIC-ACIDS

被引：14

作者：

KOBAYASHI, M ^{[1
]}

ICHISHIMA, E ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC AGR,DEPT AGR CHEM,SENDAI,MIYAGI 981,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 189卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90056-F

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reaction between glucuronic acid and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was monitored by the o-phthalaldehyde (OPA) method, which was developed for the fluorescent assay of compounds containing an amino group. About 1 nmol of glucuronic acid was detected by this method. This EDC-OPA method was effective in detecting not only acidic sugar but also carboxylic acid. Although the sensitivity of the EDC-OPA method was somewhat lower than that of amino acid determination by OPA, a very simple and convenient assay was attained for compounds containing a carboxyl group. © 1990.

引用

页码：122 / 125

页数：4

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